MRPL38 Monoclonal antibody

MRPL38 Monoclonal Antibody for WB, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1D11A1

Cat no : 68418-1-Ig

Synonyms

FLJ13996, HSPC262, KIAA1863, L38mt, MRP L3, MRP L38, MRPL38, RPML3

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Validation Data Gallery

• 

  WB analysis using 68418-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68418-1-Ig (MRPL38 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis of pig liver using 68418-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68418-1-Ig (MRPL38 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 68418-1-Ig

  IP result of anti-MRPL38 (IP:68418-1-Ig, 4ug; Detection:68418-1-Ig 1:2000) with A549 cells lysate 920 ug.

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Tested Applications

• Positive WB detected in: A549 cells, pig liver tissue, LNCaP cells, Jurkat cells, K-562 cells
• Positive IP detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68418-1-Ig targets MRPL38 in WB, IP, ELISA applications and shows reactivity with Human, pig samples.

• Tested Reactivity: Human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MRPL38 fusion protein Ag8927
• Full Name: mitochondrial ribosomal protein L38
• Calculated Molecular Weight: 346 aa, 41 kDa
• Observed Molecular Weight: 37-45 kDa
• GenBank Accession Number: BC013311
• Gene Symbol: MRPL38
• Gene ID (NCBI): 64978
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.