MRPL23 Polyclonal antibody

MRPL23 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 11706-1-AP

Synonyms

L23MRP, L23mt, MRP L23, MRPL23, Ribosomal protein L23 like, RPL23, RPL23L

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Validation Data Gallery

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  WB analysis of A2780 using 11706-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11706-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11706-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11706-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11706-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11706-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11706-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 11706-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 11706-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11706-1-AP (MRPL23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11706-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11706-1-AP (MRPL23 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 11706-1-AP

  Immunofluorescent analysis of PC-3 cells using 11706-1-AP (MRPL23 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 11706-1-AP

  Immunofluorescent analysis of MCF-7 cells using 11706-1-AP (MRPL23 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A2780 cells, HEK-293 cells, HeLa cells, MCF-7 cells, PC-3 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: PC-3 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below

Product Information

11706-1-AP targets MRPL23 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPL23 fusion protein Ag2220
• Full Name: mitochondrial ribosomal protein L23
• Calculated Molecular Weight: 153 aa, 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC027710
• Gene Symbol: MRPL23
• Gene ID (NCBI): 6150
• RRID: AB_10694422
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice.; Authors - Tara R Richman
human	WB	Nucleic Acids Res; METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis.; Authors - Lindsey Van Haute
mouse	WB	Aging Cell; Mitochondrial mistranslation modulated by metabolic stress causes cardiovascular disease and reduced lifespan.; Authors - Tara R Richman
mouse	WB	J Cell Biol; Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.; Authors - Uwe Richter
mouse	WB	PLoS Genet; Mutation in MRPS34 Compromises Protein Synthesis and Causes Mitochondrial Dysfunction.; Authors - Tara R Richman
human	WB	Genomics Proteomics Bioinformatics; Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses.; Authors - Ruona Shi