MOS Polyclonal antibody

MOS Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26454-1-AP

Synonyms

c-mos, MOS, MSV, oncogene MOS, Oocyte maturation factor mos, Proto oncogene c Mos

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Validation Data Gallery

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  WB analysis using 26454-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26454-1-AP (MOS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26454-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26454-1-AP (MOS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 26454-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26454-1-AP (MOS Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 26454-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26454-1-AP (MOS Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 26454-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MOS antibody (26454-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HEK-293 cells, Jurkat cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26454-1-AP targets MOS in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MOS fusion protein Ag24585
• Full Name: v-mos Moloney murine sarcoma viral oncogene homolog
• Calculated Molecular Weight: 346 aa, 38 kDa
• Observed Molecular Weight: 34-38 kDa
• GenBank Accession Number: BC069569
• Gene Symbol: MOS
• Gene ID (NCBI): 4342
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mos is a germ cell-specific serine/threonine protein kinase, can induce oncogenic transformation of somatic cells by direct phosphorylation of MAP kinase/ERK kinase (MEK1), activating the mitogen-activated protein kinases ERK1 and ERK2.