MGME1 Monoclonal antibody

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• KD/KO Validated

MGME1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2C4G1

Cat no : 67468-1-Ig

Synonyms

bA504H3.4, C20orf72, FLJ14597, MGME1

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Validation Data Gallery

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  WB analysis using 67468-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67468-1-Ig (MGME1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67468-1-Ig

  WB result of MGME1 antibody (67468-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MGME1 transfected HeLa cells.

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  WB analysis of HL-60 using 67468-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67468-1-Ig (MGME1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 67468-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67468-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67468-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67468-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67468-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67468-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HL-60 cells, HepG2 cells, LNCaP cells, A549 cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human lung cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67468-1-Ig targets MGME1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MGME1 fusion protein Ag19644
• Full Name: chromosome 20 open reading frame 72
• Calculated Molecular Weight: 344 aa, 39 kDa
• Observed Molecular Weight: 36-39 kDa
• GenBank Accession Number: BC016869
• Gene Symbol: C20orf72
• Gene ID (NCBI): 92667
• RRID: AB_2882697
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Aging (Albany NY); MGME1 associates with poor prognosis and is vital for cell proliferation in lower-grade glioma; Authors - Feng Xiao