MEA1 Polyclonal antibody

MEA1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10267-1-AP

Synonyms

HYS, male enhanced antigen 1, MEA, MEA 1, MEA1

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Validation Data Gallery

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  WB analysis of Jurkat using 10267-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10267-1-AP (MEA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10267-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10267-1-AP (MEA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 10267-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10267-1-AP (MEA1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse testis tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10267-1-AP targets MEA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MEA1 fusion protein Ag0375
• Full Name: male-enhanced antigen 1
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 34 kDa and 36 kDa
• GenBank Accession Number: BC001754
• Gene Symbol: MEA1
• Gene ID (NCBI): 4201
• RRID: AB_2143838
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEA1, also named Male-enhanced antigen 1, is a 185 amino acid protein, which is highly expressed in testis. MEA1 may play an important role in spermatogenesis and/or testis development. Endogenous protein was detected as two bands (34 and 36 kDa). (PMID: 12444059)