MCM8 Polyclonal antibody

MCM8 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 16451-1-AP

Synonyms

C20orf154, MCM8, Minichromosome maintenance 8, REC

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Validation Data Gallery

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  WB analysis using 16451-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16451-1-AP (MCM8 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 16451-1-AP

  IP result of anti-MCM8 (IP:16451-1-AP, 4ug; Detection:16451-1-AP 1:300) with NIH/3T3 cells lysate 4000ug.

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Tested Applications

• Positive WB detected in: HEK-293T cells, NIH/3T3 cells
• Positive IP detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

16451-1-AP targets MCM8 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MCM8 fusion protein Ag8696
• Full Name: minichromosome maintenance complex component 8
• Calculated Molecular Weight: 880 aa, 98 kDa
• Observed Molecular Weight: 95 kDa
• GenBank Accession Number: BC008830
• Gene Symbol: MCM8
• Gene ID (NCBI): 84515
• RRID: AB_2142833
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCM8, also named as Minichromosome maintenance 8, is a 840 amino acid protein, which belongs to the MCM family. MCM8 is expressed highly in placenta, lung and pancreas and various tumors with highest levels in colon and lung cancers. MCM8 is a component of the MCM8-MCM9 complex, which involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. MCM8 exists some isoforms with MV 94 kDa and 70 kDa.

Publications

Species	Application	Title
human		Nat Commun; DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain.; Authors - Jing Zhang
human	WB	Nat Commun; MCM8IP activates the MCM8-9 helicase to promote DNA synthesis and homologous recombination upon DNA damage.; Authors - Jen-Wei Huang
human	WB	Cell Death Dis; Aberrantly expressed HORMAD1 disrupts nuclear localization of MCM8-MCM9 complex and compromises DNA mismatch repair in cancer cells.; Authors - Kang Liu
human	WB	Res Sq; Mechanism of DNA unwinding by hexameric MCM8-9 in complex with HROB; Authors - Ananya Acharya
human	WB	Nat Commun; A multi-functional role for the MCM8/9 helicase complex in maintaining fork integrity during replication stress; Authors - Wezley C. Griffin
human	WB,IF,CoIP	J Cell Biol; REC drives recombination to repair double-strand breaks in animal mtDNA; Authors - Anna Klucnika