MBD3 Polyclonal antibody

• Featured Product
• KD/KO Validated

MBD3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ChIP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 14258-1-AP

Synonyms

MBD3

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Validation Data Gallery

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  WB analysis of Y79 using 14258-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 14258-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14258-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14258-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14258-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 14258-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14258-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 14258-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14258-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14258-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14258-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 14258-1-AP

  IP result of anti-MBD3 (IP:14258-1-AP, 4ug; Detection:14258-1-AP 1:800) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human endometrial cancer using 14258-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14258-1-AP (MBD3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human endometrial cancer using 14258-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14258-1-AP (MBD3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 14258-1-AP

  Immunofluorescent analysis of HepG2 cells, using MBD3 antibody 14258-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HepG2 using 14258-1-AP

  Immunofluorescent analysis of HepG2 cells, using MBD3 antibody 14258-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: Y79 cells, COLO 320 cells, HeLa cells, Jurkat cells, MCF-7 cells, mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human endometrial cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Product Information

14258-1-AP targets MBD3 in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MBD3 fusion protein Ag5531
• Full Name: methyl-CpG binding domain protein 3
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC043619
• Gene Symbol: MBD3
• Gene ID (NCBI): 53615
• RRID: AB_2139745
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MBD3 does not bind DNA by itself. It recruits histone deacetylases and DNA methyltransferases. MBD3 acts as transcriptional repressor and plays a role in gene silencing.(PMID:9774669) .

Publications

Species	Application	Title
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang ; KD Validated
		Dev Cell; Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells; Authors - Wenxiu Dai
human	WB,IF	Proc Natl Acad Sci U S A; Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development.; Authors - Ma Pengpeng P
human	WB,IHC	Cancers (Basel); MBD3 as a Potential Biomarker for Colon Cancer: Implications for Epithelial-Mesenchymal Transition (EMT) Pathways; Authors - Yuntao Ding
human	WB,ChIP	Oncol Lett; Demethylation of HACE1 gene promoter by propofol promotes autophagy of human A549 cells.; Authors - Shanshan Li
human	WB	Genet Test Mol Biomarkers; The Expression of MBD6 Is Associated with Tumor Size in Uterine Leiomyomas.; Authors - Mei-Rong Liang