SIN1 Polyclonal antibody

SIN1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 15463-1-AP

Synonyms

JC310, MAPKAP1, MIP1, mSIN1, SAPK interacting protein 1, SIN1, SIN1b, SIN1g, TORC2 subunit MAPKAP1

---

Validation Data Gallery

• 

  WB analysis of mouse kidney using 15463-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15463-1-AP (MAPKAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15463-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15463-1-AP (MAPKAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 15463-1-AP

  IP result of anti-SIN1 (IP:15463-1-AP, 4ug; Detection:15463-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human kidney using 15463-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15463-1-AP (MAPKAP1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15463-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15463-1-AP (MAPKAP1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse kidney using 15463-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15463-1-AP (SIN1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 15463-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MAPKAP1 antibody (15463-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: mouse kidney tissue, HeLa cells
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: mouse kidney tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

15463-1-AP targets SIN1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIN1 fusion protein Ag7753
• Full Name: mitogen-activated protein kinase associated protein 1
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC002326
• Gene Symbol: MAPKAP1
• Gene ID (NCBI): 79109
• RRID: AB_10598466
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Cell Rep; Mitochondrial dynamics define muscle fiber type by modulating cellular metabolic pathways; Authors - Tatsuki Yasuda
mouse	WB	bioRxiv; Host-derived oxidized phospholipids initiate effector-triggered immunity fostering lethality upon microbial encounter; Authors - Marco Di Gioia
mouse	WB	Cell Rep; Hepatic DNAJB9 Drives Anabolic Biasing to Reduce Steatosis and Obesity.; Authors - Fangfang Sun
mouse	WB	Nat Biotechnol; Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution; Authors - Qing Dai
human	WB	Mol Cell; The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.; Authors - Joanna R Kovalski