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LMX1A Polyclonal antibody

LMX1A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14144-1-AP

Synonyms

LIM/homeobox protein 1.1, LIM/homeobox protein LMX1A, LMX 1, LMX 1.1, LMX1, LMX1.1, LMX1A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LMX1A Polyclonal antibody (14144-1-AP)

    WB analysis using 14144-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14144-1-AP (LMX1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14144-1-AP targets LMX1A in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LMX1A fusion protein Ag5311
Full Name LIM homeobox transcription factor 1, alpha
Calculated Molecular Weight 43 kDa
Observed Molecular Weight43 kDa
GenBank Accession NumberBC066353
Gene Symbol LMX1A
Gene ID (NCBI) 4009
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LMX1A is a transcription factor that positively regulates insulin gene transcription. LMX1A also plays a role in the development of dopamine-producing neurons during embryogenesis (PMID: 36381759). LMX1A has 2 isoforms with the molecular mass of 15 kDa (LMX1A-4AB) and 43 kDa (Isoform 1). LMX1A-4AB is expressed in testis. Isoform 1 of LMX1A is expressed in many tissues but not found in heart, liver, spleen and testis.

Protocols

Product Specific Protocols
WB protocol for LMX1A antibody 14144-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols