KIF19 Polyclonal antibody

KIF19 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 19551-1-AP

Synonyms

KIF19, KIF19A, kinesin family member 19, Kinesin like protein KIF19

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Validation Data Gallery

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  WB analysis of human brain using 19551-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19551-1-AP (KIF19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 19551-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19551-1-AP (KIF19 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 19551-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19551-1-AP (KIF19 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 19551-1-AP

  Immunofluorescent analysis of HepG2 cells, using KIF19 antibody 19551-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

19551-1-AP targets KIF19 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: kinesin family member 19
• Calculated Molecular Weight: 111 kDa
• Observed Molecular Weight: 92 kDa
• GenBank Accession Number: NM_153209
• Gene Symbol: KIF19
• Gene ID (NCBI): 124602
• RRID: AB_10640802
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIF19 belongs to the kinesin-like protein family. The antibody recognizes isoform1 of KIF19 only.

Publications

Species	Application	Title
mouse	WB	J Mol Cell Biol; Production of transgenic mice by random recombination of targeted genes in female germline stem cells.; Authors - Zhang Yong Y
human,mouse	WB,IF,IP	J Biol Chem; AC6 regulates the microtubule-depolymerizing kinesin KIF19A to control ciliary length in mammals.; Authors - Kavisha Arora