CoraLite® Plus 488-conjugated KGA-Specific Monoclonal antibody

KGA-Specific Monoclonal Antibody for IF, WB

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B11A11

Cat no : CL488-66265

Synonyms

AAD20, DKFZp686O15119, FLJ10358, GAC, GLS, GLS1, glutaminase, K glutaminase, KGA-Specific, KIAA0838, L glutamine amidohydrolase

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Validation Data Gallery

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  WB analysis using CL488-66265

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66265 (KGA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using CL488-66265

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 KGA-Specific antibody (CL488-66265, Clone: 1B11A11 ) at dilution of 1:200.

• 

  IF Staining of mouse brain using CL488-66265

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 KGA-Specific antibody (CL488-66265, Clone: 1B11A11 ) at dilution of 1:200.

• 

  IF Staining of mouse brain using CL488-66265

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 KGA-Specific antibody (CL488-66265, Clone: 1B11A11 ) at dilution of 1:200.

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, mouse brain tissue
• Positive IF detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66265 targets KGA-Specific in WB, IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: KGA-Specific fusion protein Ag24227
• Full Name: glutaminase
• Calculated Molecular Weight: 669 aa, 73 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC038507
• Gene Symbol: GLS
• Gene ID (NCBI): 2744
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID: 12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID: 447624;7512428). It has 3 isoforms produced by alternative splicing named as KGA, GAM, GAC. This antibody is specific to KGA.