KCTD2/5/17 Polyclonal antibody

• Featured Product
• KD/KO Validated

KCTD2/5/17 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 15553-1-AP

Synonyms

KCTD17, KCTD2, KCTD5

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Validation Data Gallery

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  WB analysis of HeLa using 15553-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15553-1-AP (KCTD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15553-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15553-1-AP (KCTD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15553-1-AP

  IP result of anti-KCTD2/5/17 (IP:15553-1-AP, 4ug; Detection:15553-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of human ovary tumor using 15553-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15553-1-AP (KCTD5 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 15553-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15553-1-AP (KCTD5 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 15553-1-AP

  Immunofluorescent analysis of Hela cells, using KCTD5 antibody 15553-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 15553-1-AP

  1X10^6 HeLa cells were stained with 0.2ug KCTD5 antibody (15553-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IP: See 1 publications below

Product Information

15553-1-AP targets KCTD2/5/17 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KCTD2/5/17 fusion protein Ag7915
• Full Name: potassium channel tetramerisation domain containing 5
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC007314
• Gene Symbol: KCTD5
• Gene ID (NCBI): 54442
• RRID: AB_2132155
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KCTD5 belongs to the human potassium (K+) channel tetramerization domain (KCTD) family of proteins which share sequence similarity with the cytoplasmic domain of voltage-gated K+ channels (Kv channels). The KCTD proteins have relatively conserved N-terminal domains and variable C-termini (PMID: 24268103). KCTD5 interacts with cullin3 and has been proposed to function as substrate adapter for cullin3 based ubiquitin E3 ligases (PMID: 26188516). This polyclonal antibody raised against full-length recombinant protein of human KCTD5 (26 kDa) can cross-react with other KCTD proteins, including KCTD2 (29 kDa), KCTD17 (33-36 kDa).

Publications

Species	Application	Title
human	WB	Sci Adv; Structural basis for the ubiquitination of G protein βγ subunits by KCTD5/Cullin3 E3 ligase; Authors - Wentong Jiang
human	WB	Nat Commun; Knowledge graph-based recommendation framework identifies drivers of resistance in EGFR mutant non-small cell lung cancer.; Authors - Anna Gogleva ; KO Validated
human	WB	Diabetes; HOXC10 Suppresses Browning to Maintain White Adipocyte Identity.; Authors - H Y Angeline Tan
human	WB	Elife; Genome-wide CRISPR screening reveals genetic modifiers of mutant EGFR dependence in human NSCLC.; Authors - Hao Zeng
rat	WB	PLoS Genet; Conserved properties of Drosophila Insomniac link sleep regulation and synaptic function.; Authors - Qiuling Li
human	WB,IP	J Proteome Res; Human Multisubunit E3 Ubiquitin Ligase Required for Heterotrimeric G-Protein β-Subunit Ubiquitination and Downstream Signaling.; Authors - Brian D Young ; KD Validated