INPPL1 Polyclonal antibody

INPPL1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26678-1-AP

Synonyms

INPPL 1, INPPL1, Protein 51C, SHIP 2, SHIP2

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Validation Data Gallery

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  WB analysis using 26678-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26678-1-AP (INPPL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 26678-1-AP

  IP result of anti-INPPL1 (IP:26678-1-AP, 4ug; Detection:26678-1-AP 1:800) with HeLa cells lysate 2520 ug.

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  IHC staining of mouse stomach using 26678-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26678-1-AP (INPPL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 26678-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26678-1-AP (INPPL1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26678-1-AP targets INPPL1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: INPPL1 fusion protein Ag24790
• Full Name: inositol polyphosphate phosphatase-like 1
• Calculated Molecular Weight: 1258 aa, 139 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC140853
• Gene Symbol: INPPL1
• Gene ID (NCBI): 3636
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.