HSPB11 Monoclonal antibody

HSPB11 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D11B3

Cat no : 68059-1-Ig

Synonyms

C1orf41, Heat shock protein beta 11, HSPB11, HSPCO34, ITF25, Placental protein 25, PP25

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Validation Data Gallery

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  WB analysis using 68059-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68059-1-Ig (HSPB11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: NCCIT cells, LNCaP cells, HeLa cells, HEK-293 cells, T-47D cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68059-1-Ig targets HSPB11 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: HSPB11 fusion protein Ag8804
• Full Name: heat shock protein family B (small), member 11
• Calculated Molecular Weight: 144 aa, 16 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC005245
• Gene Symbol: HSPB11
• Gene ID (NCBI): 51668
• RRID: AB_2918800
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSPB11 (Heat shock protein beta-11), also known as IFT25, was originally identified as a small heat shock protein. Recently it has been identified as a component of IFT complex B. It has been demonstrated that IFT25 is not required for ciliary assembly but is required for proper Hedgehog signaling, which in mammals occurs within cilia. Cilia lacking IFT25 have defects in the signal-dependent transport of multiple Hedgehog components and fail to activate the pathway upon stimulation.