Hephaestin Polyclonal antibody

Hephaestin Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11148-1-AP

Synonyms

CPL, HEPH, Hephaestin, KIAA0698

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Validation Data Gallery

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  IHC staining of human pancreas cancer using 11148-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11148-1-AP (Hephaestin antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

11148-1-AP targets Hephaestin in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Hephaestin fusion protein Ag1604
• Full Name: hephaestin
• Calculated Molecular Weight: 130 kDa
• GenBank Accession Number: BC011561
• Gene Symbol: HEPH
• Gene ID (NCBI): 9843
• RRID: AB_11064728
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hephaestin, also named as KIAA0698, belongs to the multicopper oxidase family. It is provied as ferroxidase of cellular iron export. HEPH is implicated in iron homeostasis and may mediate iron efflux associated to ferroportin 1. HEPE is active in placenta but may not play a key role in placental iron transport.

Publications

Species	Application	Title
human	WB	Cell Biochem Funct; Ferroportin 1 and hephaestin expression in BeWo cell line with different iron treatment.; Authors - Li Yan-Qin YQ
rat	WB	Chin Herb Med; Neuroprotective effects of kukoamine A on 6-OHDA-induced Parkinson's model through apoptosis and iron accumulation inhibition; Authors - Xin Li