CoraLite®594-conjugated Glycophorin A Monoclonal antibody

Glycophorin A Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2B12G10

Cat no : CL594-66778

Synonyms

CD235a, Glycophorin A, GPA, GPErik, GpMiIII, GPSAT, GYPA, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MN sialoglycoprotein, MNS, PAS 2, Sialoglycoprotein alpha

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Validation Data Gallery

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  IF Staining of human placenta using CL594-66778

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite®594 Glycophorin A antibody (CL594-66778, Clone: 2B12G10 ) at dilution of 1:200.

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  IF Staining of human placenta using CL594-66778

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite®594 Glycophorin A antibody (CL594-66778, Clone: 2B12G10 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human placenta tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66778 targets Glycophorin A in IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Glycophorin A fusion protein Ag8635
• Full Name: glycophorin A (MNS blood group)
• Calculated Molecular Weight: 150 aa, 16 kDa
• Observed Molecular Weight: 36-38 kDa
• GenBank Accession Number: BC005319
• Gene Symbol: GYPA
• Gene ID (NCBI): 2993
• RRID: AB_2920035
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)