GYG2 Polyclonal antibody

GYG2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13150-1-AP

Synonyms

glycogenin 2, GN 2, GN2, GYG2

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Validation Data Gallery

• 

  WB analysis of L02 using 13150-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 13150-1-AP (GYG2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13150-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13150-1-AP (GYG2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13150-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13150-1-AP (GYG2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: L02 cells, HEK-293 cells, human liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13150-1-AP targets GYG2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GYG2 fusion protein Ag3765
• Full Name: glycogenin 2
• Calculated Molecular Weight: 501 aa, 55 kDa
• Observed Molecular Weight: 45 kDa, 66-70 kDa
• GenBank Accession Number: BC023152
• Gene Symbol: GYG2
• Gene ID (NCBI): 8908
• RRID: AB_2116111
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GYG2 belongs to the glycogenin family. Glycogenin is a self-glucosylating protein involved in the initiation phase of glycogen biosynthesis. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, including the predominant band around 66 kDa and a light band around 45 kDa.