GSTM5 Polyclonal antibody

GSTM5 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 14502-1-AP

Synonyms

glutathione S transferase mu 5, GST class mu 5, GSTM5, GSTM5 5, GTM5

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Validation Data Gallery

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  WB analysis using 14502-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14502-1-AP (GSTM5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skeletal muscle using 14502-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14502-1-AP (GSTM5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 14502-1-AP

  IP result of anti-GSTM5 (IP:14502-1-AP, 4ug; Detection:14502-1-AP 1:600) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of human heart using 14502-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14502-1-AP (GSTM5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 14502-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14502-1-AP (GSTM5 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 14502-1-AP

  Immunofluorescent analysis of HepG2 cells, using GSTM5 antibody 14502-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HeLa cells, rat skeletal muscle tissue, mouse testis
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

14502-1-AP targets GSTM5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSTM5 fusion protein Ag5958
• Full Name: glutathione S-transferase mu 5
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC058881
• Gene Symbol: GSTM5
• Gene ID (NCBI): 2949
• RRID: AB_2263888
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSTM5 belongs to the GST superfamily that metabolizes a broad range of xenobiotics and carcinogens. GSTM5 is expressed in brain, testis, lung and a lesser extent in heart.

Publications

Species	Application	Title
mouse	WB	J Proteomics; Quantitative proteomic analysis of oxaliplatin induced peripheral neurotoxicity.; Authors - Linlin Yang
rat	WB, IHC, IF	J Neurol Sci; Proteomic analysis of the hippocampus in naïve and ischemic-preconditioned rat.; Authors - Takayuki Nakajima
mouse	WB	Andrology; Thiol changes during epididymal maturation: a link to flagellar angulation in mouse spermatozoa?; Authors - Ijiri T W TW