GSTM4 Polyclonal antibody

GSTM4 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 16766-1-AP

Synonyms

glutathione S transferase mu 4, GST class mu 4, GST Mu2, GSTM4, GSTM4 4, GTM4

---

Validation Data Gallery

• 

  WB analysis of mouse liver using 16766-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16766-1-AP (GSTM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16766-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16766-1-AP (GSTM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16766-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16766-1-AP (GSTM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 16766-1-AP

  IP result of anti-GSTM4 (IP:16766-1-AP, 4ug; Detection:16766-1-AP 1:500) with mouse lung tissue lysate 4000 ug.

• 

  IHC staining of human cervical cancer using 16766-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16766-1-AP (GSTM4 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 16766-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16766-1-AP (GSTM4 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16766-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16766-1-AP (GSTM4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: mouse liver tissue, HeLa cells, mouse lung tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

16766-1-AP targets GSTM4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSTM4 fusion protein Ag10340
• Full Name: glutathione S-transferase mu 4
• Calculated Molecular Weight: 218 aa, 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC015513
• Gene Symbol: GSTM4
• Gene ID (NCBI): 2948
• RRID: AB_2116072
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	J Gerontol A Biol Sci Med Sci; PMID: 24285747; Authors - Rojanathammanee Lalida L
mouse	WB	Age (Dordr); PMID: 25001375; Authors - Lalida Rojanathammanee
human, mouse	WB	Mutat Res; Black soybean seed coat polyphenols prevent B(a)P-induced DNA damage through modulating drug-metabolizing enzymes in HepG2 cells and ICR mice.; Authors - Zhang Tianshun T
human	WB,IHC	Reprod Sci; 6-(7-Nitro-2,1,3-benzoxadiazol-4-ylthio) Hexanol Inhibits Proliferation and Induces Apoptosis of Endometriosis by Regulating Glutathione S-Transferase Mu Class 4; Authors - Wei Liu