GSG1 Polyclonal antibody

GSG1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 15258-1-AP

Synonyms

germ cell associated 1, GSG1

---

Validation Data Gallery

• 

  WB analysis using 15258-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15258-1-AP (GSG1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse testis using 15258-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using GSG1 antibody (15258-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IF detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15258-1-AP targets GSG1 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSG1 fusion protein Ag7200
• Full Name: germ cell associated 1
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC001796
• Gene Symbol: GSG1
• Gene ID (NCBI): 83445
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSG1 causes the redistribution of PAPOLB from the cytosol to the endoplasmic reticulum. It has eight isoforms with MW 31-33 kDa and 36-40 kDa.