GPATCH4 Polyclonal antibody

GPATCH4 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24399-1-AP

Synonyms

G patch domain containing 4, GPATC4, GPATCH4

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Validation Data Gallery

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  WB analysis of HEK-293 using 24399-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24399-1-AP (GPATCH4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 24399-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24399-1-AP (GPATCH4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 24399-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 24399-1-AP (GPATCH4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24399-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24399-1-AP (GPATCH4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 24399-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 24399-1-AP (GPATCH4 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver cancer using 24399-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 24399-1-AP (GPATCH4 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 24399-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 24399-1-AP (GPATCH4 Antibody) at dilution of 1:50.

• 

  IF Staining of HEK-293 using 24399-1-AP

  Immunofluorescent analysis of HEK-293 cells using 24399-1-AP (GPATCH4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: human liver tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24399-1-AP is GPATCH4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPATCH4 fusion protein Ag20095
• Full Name: G patch domain containing 4
• Calculated Molecular Weight: 446 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC040147
• Gene Symbol: GPATCH4
• Gene ID (NCBI): 54865
• RRID: AB_2879523
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPATCH4 (G patch domain-containing protein 4) is a 446 amino acid protein containing one G-patch domain. Existing as three alternatively spliced isoforms. This antibody is a rabbit polyclonal antibody raised against the 189 residues of N-terminal of human GPATCH4 protein