GLO1 Monoclonal antibody

GLO1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B7A8

Cat no : 68242-1-Ig

Synonyms

Aldoketomutase, GLO1, GLOD1, Glx I, GLYI, glyoxalase I, Ketone aldehyde mutase, Lactoylglutathione lyase, Methylglyoxalase

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Validation Data Gallery

• 

  WB analysis using 68242-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68242-1-Ig (GLO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 68242-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68242-1-Ig (GLO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68242-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68242-1-Ig (GLO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, LNCaP cells, Jurkat cells, HepG2 cells, K-562 cells, HSC-T6 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68242-1-Ig targets GLO1 in WB, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

• Tested Reactivity: Human, Rat, Mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: GLO1 fusion protein Ag7314
• Full Name: glyoxalase I
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21-25 kDa
• GenBank Accession Number: BC001741
• Gene Symbol: GLO1
• Gene ID (NCBI): 2739
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glo1 (glyxoalase I) is a cytosolic protein expressed in all mammalian cells. Its physiological function is the detoxification of MG (methylglyoxal), which is a potent precursor of AGEs (advanced glycation end-products). Glo1 is known to play a role in many diseases including diabetes mellitus, Alzheimer's disease, and cancer.