GAGE2D Polyclonal antibody

GAGE2D Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12532-1-AP

Synonyms

Cancer/testis antigen 4.8, CT4.8, G antigen 2D, G antigen 8, GAGE 8, GAGE2D, GAGE8

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Validation Data Gallery

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  WB analysis of K-562 using 12532-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12532-1-AP (GAGE2D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 12532-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12532-1-AP (GAGE2D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12532-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12532-1-AP (GAGE2D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 12532-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12532-1-AP (GAGE2D Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 12532-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12532-1-AP (GAGE2D Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 12532-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12532-1-AP (GAGE2D Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12532-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12532-1-AP (GAGE2D Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 12532-1-AP

  Immunofluorescent analysis of HepG2 cells, using GAGE2D antibody 12532-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells, human testis tissue
• Positive IHC detected in: human liver cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12532-1-AP targets GAGE2D in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GAGE2D fusion protein Ag3230
• Full Name: G antigen 2D
• Calculated Molecular Weight: 116 aa, 13 kDa
• Observed Molecular Weight: 18-22 kDa
• GenBank Accession Number: BC018052
• Gene Symbol: GAGE2D
• Gene ID (NCBI): 729408
• RRID: AB_2877864
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GAGE2D, also named as GAGE8 and CT4.8, is expressed in testis and tumor tissues.