FOXRED1 Polyclonal antibody

• Featured Product
• KD/KO Validated

FOXRED1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 24595-1-AP

Synonyms

FOXRED1, FP634, H17

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Validation Data Gallery

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  WB analysis of HeLa using 24595-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24595-1-AP (FOXRED1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 24595-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24595-1-AP (FOXRED1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 24595-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24595-1-AP (FOXRED1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 24595-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24595-1-AP (FOXRED1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below

Product Information

The immunogen of 24595-1-AP is FOXRED1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FOXRED1 fusion protein Ag20138
• Full Name: FAD-dependent oxidoreductase domain containing 1
• Calculated Molecular Weight: 486 aa, 54 kDa
• Observed Molecular Weight: 30 kDa, 50 kDa
• GenBank Accession Number: BC013902
• Gene Symbol: FOXRED1
• Gene ID (NCBI): 55572
• RRID: AB_2879629
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cell Biol; A mutation in the FAD-dependent oxidoreductase FOXRED1 results in cell-type specific assembly defects in oxidative phosphorylation complexes I and II.; Authors - Olga Zurita Rendón ; KD Validated
human	WB	J Clin Med; Identification and Characterization of New Variants in FOXRED1 Gene Expands the Clinical Spectrum Associated with Mitochondrial Complex I Deficiency.; Authors - Sofia Barbosa-Gouveia
human	WB	Cell Rep; A membrane arm of mitochondrial complex I sufficient to promote respirasome formation.; Authors - Hezhi Fang ; KD Validated
human	WB	iScience; Deficient tRNA posttranscription modification dysregulated the mitochondrial quality controls and apoptosis; Authors - Yunfan He