FKBP2 Monoclonal antibody

FKBP2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2H7E8

Cat no : 66091-1-Ig

Synonyms

13 kDa FK506 binding protein, 13 kDa FKBP, FK506 binding protein 2, FK506 binding protein 2, 13kDa, FKBP 13, FKBP 2, FKBP13, FKBP2, Immunophilin FKBP13, PPIase, PPIase FKBP2, Rotamase

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Validation Data Gallery

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  WB analysis of human cerebellum using 66091-1-Ig

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 66091-1-Ig (FKBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 66091-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66091-1-Ig (FKBP2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 66091-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66091-1-Ig (FKBP2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 66091-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66091-1-Ig (FKBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 66091-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66091-1-Ig (FKBP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66091-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66091-1-Ig (FKBP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Human cerebellum tissue, human brain tissue, pig liver tissue, rat brain tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66091-1-Ig targets FKBP2 in WB, IHC, ELISA applications and shows reactivity with human, rat, pig samples.

• Tested Reactivity: human, rat, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FKBP2 fusion protein Ag19113
• Full Name: FK506 binding protein 2, 13kDa
• Calculated Molecular Weight: 142 aa, 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC003384
• Gene Symbol: FKBP2
• Gene ID (NCBI): 2286
• RRID: AB_11232416
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FKBP2 is also named as FKBP13 and belongs to the FKBP-type PPIase family. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. FKBP2 has a 21-amino acid signal peptide and appears to be membrane-associated(PMID:1713687). It is localized to the lumen of the endoplasmic reticulum (ER). FKBP12 and FKBP13 are highly similar proteins, of molecular masses 12 kDa and 13 kDa respectively, with approx.43 % amino acid identity. The strong homology between FKBP12 and FKBP13 suggests that they may share similar biological functions, although, apart from rotamase activity, details relating to the function of either protein are scant(PMID:8373365).

Publications

Species	Application	Title
human	WB	Emerg Microbes Infect; Pharmacological suppression of glycogen synthase kinase-3 reactivates HIV-1 from latency via activating Wnt/β-catenin/TCF1 axis in CD4+ T cells.; Authors - Jing Wen