FAM78A Polyclonal antibody

FAM78A Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21038-1-AP

Synonyms

C9orf59, FAM78A, Protein FAM78A

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Validation Data Gallery

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  WB analysis of mouse liver using 21038-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21038-1-AP (FAM78A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 21038-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21038-1-AP (FAM78A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 21038-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 21038-1-AP (FAM78A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21038-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21038-1-AP (FAM78A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 21038-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21038-1-AP (FAM78A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 21038-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 21038-1-AP (FAM78A Antibody) at dilution of 1:50.

• 

  IHC staining of human tonsillitis using 21038-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 21038-1-AP (FAM78A Antibody) at dilution of 1:50.

• 

  IHC staining of human colon cancer using 21038-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 21038-1-AP (FAM78A Antibody) at dilution of 1:50.

• 

  IHC staining of human colon cancer using 21038-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 21038-1-AP (FAM78A Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 21038-1-AP

  Immunofluorescent analysis of HepG2 cells using 21038-1-AP (FAM78A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse liver tissue, HepG2 cells, human liver tissue, mouse spleen tissue
• Positive IHC detected in: human tonsillitis tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21038-1-AP is FAM78A Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM78A fusion protein Ag15366
• Full Name: family with sequence similarity 78, member A
• Calculated Molecular Weight: 283 aa, 32 kDa
• Observed Molecular Weight: 32-35 kDa
• GenBank Accession Number: BC049388
• Gene Symbol: FAM78A
• Gene ID (NCBI): 286336
• RRID: AB_10973677
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.