FAM3C Polyclonal antibody

FAM3C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 14247-1-AP

Synonyms

FAM3C, GS3786, ILEI, Interleukin like EMT inducer, Protein FAM3C

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Validation Data Gallery

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  WB analysis using 14247-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14247-1-AP (FAM3C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14247-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14247-1-AP (FAM3C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14247-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14247-1-AP (FAM3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14247-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14247-1-AP (FAM3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14247-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14247-1-AP (FAM3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 14247-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 14247-1-AP (FAM3C antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 14247-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 14247-1-AP (FAM3C antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach cancer using 14247-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14247-1-AP (FAM3C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 14247-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14247-1-AP (FAM3C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 14247-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 14247-1-AP (FAM3C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 14247-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 14247-1-AP (FAM3C antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HCT 116 cells, human brain tissue, human heart tissue, HEK-293 cells, HT-29 cells, HepG2 cells
• Positive IHC detected in: human pancreas tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 3 publications below

Product Information

14247-1-AP targets FAM3C in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM3C fusion protein Ag5516
• Full Name: family with sequence similarity 3, member C
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC046932
• Gene Symbol: FAM3C
• Gene ID (NCBI): 10447
• RRID: AB_2278149
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM3C, also known as ILEI, is a secreted protein belonging to the cytokine-like protein family. FAM3C is a signaling cytokine with a GG domain, hydrophobic leader sequence and an N-terminal signal peptide. A change in expression of this protein has been noted in pancreatic cancer-derived cells. FAM3C has been involved in the epithelial to mesenchymal transition, and retinal laminar formation processes in vertebrates. Also, FAM3C may play an important role in inner ear development and thus be involved in cell differentiation and proliferation during inner ear embryogenesis.

Publications

Species	Application	Title
human	WB	Sci Adv; p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.; Authors - Xiaochao Tan
human	WB	J Proteome Res; Mining the gastric cancer secretome: identification of GRN as a potential diagnostic marker for early gastric cancer.; Authors - Loei Hendrick H
human	WB	Diagn Pathol; Prognostic significance of FAM3C in esophageal squamous cell carcinoma.; Authors - Ying-Hui Zhu
human	IHC	Int J Clin Exp Pathol; Overexpression of FAM3C protein as a novel biomarker for epithelial-mesenchymal transition and poor outcome in gastric cancer.; Authors - Shuai Yin
human,mouse	WB,IHC	Int J Biol Sci; A Novel ER Stress Mediator TMTC3 Promotes Squamous Cell Carcinoma Progression by Activating GRP78/PERK Signaling Pathway; Authors - Hongyu Yuan
human	IHC	Sci Rep; FAM3 family genes are associated with prognostic value of human cancer: a pan-cancer analysis; Authors - Qing-Tai Dong