ESCO2 Polyclonal antibody

ESCO2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 23525-1-AP

Synonyms

2410004I17Rik, ECO1 homolog 2, EFO2, ESCO2, N acetyltransferase ESCO2, RBS

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Validation Data Gallery

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  WB analysis using 23525-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23525-1-AP (ESCO2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 23525-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23525-1-AP (ESCO2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, mouse testis, rat testis

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

23525-1-AP targets ESCO2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ESCO2 fusion protein Ag18893
• Full Name: establishment of cohesion 1 homolog 2 (S. cerevisiae)
• Calculated Molecular Weight: 601 aa, 68 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC146562
• Gene Symbol: ESCO2
• Gene ID (NCBI): 157570
• RRID: AB_2879292
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ESCO2 is also known as Establishment of Sister Chromatid Cohesion N-Acetyltransferase 2, EFO2. This gene encodes a member of the family of acetyltransferases involved in the establishment of sister chromatid cohesion during S phase and postreplicative sister chromatid cohesion induced by double-strand breaks (PubMed: 15821733). Mutations in the ESCO2 gene are associated with a rare genetic disorder called Roberts syndrome or SC phocomelia syndrome (PubMed: 16380922). The loss of function or impaired activity of the ESCO2 protein due to mutations disrupts the proper cohesion of sister chromatids, leading to chromosomal abnormalities and the characteristic features of Roberts syndrome. ESCO2 can be detected as about 65-70 kDa.

Publications

Species	Application	Title
human	WB	Kaohsiung J Med Sci; Long noncoding RNA ZFPM2-AS1 regulates renal cell carcinoma progression via miR-130a-3p/ESCO2.; Authors - Gang Zhang