ERP44 Monoclonal antibody

ERP44 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A2C1

Cat no : 67426-1-Ig

Synonyms

ER protein 44, ERP44, KIAA0573, PDIA10, TXNDC4

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Validation Data Gallery

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  WB analysis using 67426-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67426-1-Ig (ERP44 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67426-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67426-1-Ig (ERP44 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 67426-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67426-1-Ig (ERP44 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67426-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67426-1-Ig (ERP44 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67426-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67426-1-Ig (ERP44 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67426-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67426-1-Ig (ERP44 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67426-1-Ig targets ERP44 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ERP44 fusion protein Ag8818
• Full Name: endoplasmic reticulum protein 44
• Calculated Molecular Weight: 406 aa, 47 kDa
• Observed Molecular Weight: 44-47 kDa
• GenBank Accession Number: BC005374
• Gene Symbol: ERP44
• Gene ID (NCBI): 23071
• RRID: AB_2882665
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TXNDC4 is also named as KIAA0573, ERP44, PDIA10, ERp44 and belongs to the thioredoxin family (TRX). It may be involved in electron transport, protein folding, response to unfolded protein, glycoprotein metabolism, secretory pathway. It may have a role in the control of oxidative protein folding in the endoplasmic reticulum.