EPS8L2 Monoclonal antibody

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• KD/KO Validated

EPS8L2 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2E3B1

Cat no : 68330-1-Ig

Synonyms

EPS8 like 2, EPS8 like protein 2, EPS8 related protein 2, EPS8L2, EPS8R2, FLJ16738, FLJ21935, FLJ22171

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Validation Data Gallery

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  WB analysis of HepG2 using 68330-1-Ig

  WB result of EPS8L2 antibody (68330-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPS8L2 transfected HepG2 cells.

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  WB analysis of A431 using 68330-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 68330-1-Ig (EPS8L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skin using 68330-1-Ig

  rat skin tissue were subjected to SDS PAGE followed by western blot with 68330-1-Ig (EPS8L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 68330-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EPS8L2 antibody (68330-1-Ig, Clone: 2E3B1 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: rat skin tissue, A431 cells, HepG2 cells, A549 cells，LNCaP cells，HeLa cells，HepG2 cells, mouse skin tissue
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68330-1-Ig targets EPS8L2 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: EPS8L2 fusion protein Ag16808
• Full Name: EPS8-like 2
• Calculated Molecular Weight: 715 aa, 81 kDa
• Observed Molecular Weight: 81-85 kDa
• GenBank Accession Number: BC002474
• Gene Symbol: EPS8L2
• Gene ID (NCBI): 64787
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPS8L2 (epidermal growth factor receptor pathway substrate 8 L2), a member of the Eps8-like protein family, is a newly identified hair bundle protein that is localized at the tips of stereocilia of both cochlear and vestibular hair cells.