EPS8L2 Polyclonal antibody

• Featured Product
• KD/KO Validated

EPS8L2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20461-1-AP

Synonyms

EPS8 like 2, EPS8 like protein 2, EPS8 related protein 2, EPS8L2, EPS8R2

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Validation Data Gallery

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  WB analysis of HepG2 using 20461-1-AP

  WB result of EPS8L2 antibody (20461-1-AP; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPS8L2 transfected HepG2 cells.

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  WB analysis using 20461-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20461-1-AP (EPS8L2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20461-1-AP

  IP result of anti-EPS8L2 (IP:20461-1-AP, 4ug; Detection:20461-1-AP 1:1000) with HeLa cells lysate 3200ug.

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  IHC staining of human lung cancer using 20461-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20461-1-AP (EPS8L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 20461-1-AP

  Immunofluorescent analysis of HeLa cells using 20461-1-AP (EPS8L2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells, HeLa cells, mouse skin tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

20461-1-AP targets EPS8L2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EPS8L2 fusion protein Ag14296
• Full Name: EPS8-like 2
• Calculated Molecular Weight: 715 aa, 81 kDa
• Observed Molecular Weight: 81-85 kDa
• GenBank Accession Number: BC002474
• Gene Symbol: EPS8L2
• Gene ID (NCBI): 64787
• RRID: AB_10665945
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EPS8L2 (epidermal growth factor receptor pathway substrate 8 L2), a member of the Eps8-like protein family, is a newly identified hair bundle protein that is localized at the tips of stereocilia of both cochlear and vestibular hair cells.

Publications

Species	Application	Title
mouse	IHC	Lab Invest; Nuclear receptor binding factor 2 (NRBF2) is required for learning and memory.; Authors - Xiaosen Ouyang
mouse	IF,WB	Cell Rep; Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer's disease; Authors - Min Yu