EML1 Polyclonal antibody

EML1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12765-1-AP

Synonyms

ELP79, EMAP, EMAP 1, EMAP1, EMAPL, EMAPL1, EML1, HuEMAP, HuEMAP 1

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Validation Data Gallery

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  WB analysis using 12765-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12765-1-AP (EML1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12765-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12765-1-AP (EML1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

12765-1-AP targets EML1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EML1 fusion protein Ag3429
• Full Name: echinoderm microtubule associated protein like 1
• Calculated Molecular Weight: 815 aa, 90 kDa
• Observed Molecular Weight: 90-92 kDa
• GenBank Accession Number: BC033043
• Gene Symbol: EML1
• Gene ID (NCBI): 2009
• RRID: AB_10644132
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EML1 is a strong candidate for the Usher syndrome type 1A gene. Usher syndromes (USHs) are a group of genetic disorders consisting of congenital deafness, retinitis pigmentosa, and vestibular dysfunction of variable onset and severity depending on the genetic type. The disease process in USHs involves the entire brain and is not limited to the posterior fossa or auditory and visual systems. The USHs are catagorized as type I (USH1A, USH1B, USH1C, USH1D, USH1E and USH1F), type II (USH2A and USH2B) and type III (USH3). The type I is the most severe form. Gene loci responsible for these three types are all mapped.

Publications

Species	Application	Title
	IF,WB	Front Cell Dev Biol; Echinoderm Microtubule Associated Protein Like 1 Is Indispensable for Oocyte Spindle Assembly and Meiotic Progression in Mice.; Authors - Hong Yin