EIF1, EIF1B Polyclonal antibody

EIF1, EIF1B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15887-1-AP

Synonyms

EIF1B, GC20

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Validation Data Gallery

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  WB analysis of L02 using 15887-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15887-1-AP (EIF1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15887-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15887-1-AP (EIF1B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15887-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15887-1-AP (EIF1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15887-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15887-1-AP (EIF1B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15887-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15887-1-AP (EIF1B Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: L02 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15887-1-AP targets EIF1, EIF1B in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EIF1, EIF1B fusion protein Ag8673
• Full Name: eukaryotic translation initiation factor 1B
• Calculated Molecular Weight: 113 aa, 13 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC006996
• Gene Symbol: EIF1B
• Gene ID (NCBI): 10289
• RRID: AB_2878196
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.