EEF1E1 Polyclonal antibody

• Featured Product
• KD/KO Validated

EEF1E1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10805-1-AP

Synonyms

AIMP3, EEF1E1, Elongation factor p18, P18

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Validation Data Gallery

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  WB analysis using 10805-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10805-1-AP (EEF1E1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10805-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10805-1-AP (EEF1E1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10805-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10805-1-AP (EEF1E1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 10805-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10805-1-AP (EEF1E1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 10805-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10805-1-AP (EEF1E1 antibody) at dilution of 1:10 (under 10x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, human testis tissue, Jurkat cells, HepG2 cells, SKOV-3 cells, mouse testis tissue, rat testis tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Product Information

10805-1-AP targets EEF1E1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EEF1E1 fusion protein Ag1092
• Full Name: eukaryotic translation elongation factor 1 epsilon 1
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC005291
• Gene Symbol: EEF1E1
• Gene ID (NCBI): 9521
• RRID: AB_2097140
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

During the translation processes, aminoacyl-tRNA synthetases (ARSs) ligate specific amino acids to tRNAs. Together with non-enzymatic proteins called ARS-interacting multifunctional proteins (AIMPs), the ARSs form a macromolecular complex [PMID:15286174]. This complex has an essential role in protein synthesis, but also in other biological processes including angiogenesis, apoptosis, and inflammation. [PMID:21789020] AIMP3 is not only associated with the multi-tRNA synthetase complex via its specific interaction with methionyl-tRNA synthetase, but also works as a tumor suppressor by interacting with ATM, the upstream kinase of p53 [PMID:18343821].

Publications

Species	Application	Title
human	WB	J Cell Mol Med; AIMP3 inhibits cell growth and metastasis of lung adenocarcinoma through activating a miR-96-5p-AIMP3-p53 axis.; Authors - Liting Ding
human	WB	Cell Rep; Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome.; Authors - J J David Ho
human	WB,IF	Proc Natl Acad Sci U S A; Multimodal cotranslational interactions direct assembly of the human multi-tRNA synthetase complex; Authors - Krishnendu Khan ; KD Validated