EDC4 Polyclonal antibody

• Featured Product
• KD/KO Validated

EDC4 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 17737-1-AP

Synonyms

Autoantigen Ge 1, Autoantigen RCD 8, EDC4, enhancer of mRNA decapping 4, Ge 1, HEDLS, RCD 8

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Validation Data Gallery

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  WB analysis of HeLa using 17737-1-AP

  WB result of EDC4 antibody (17737-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EDC4 transfected HeLa cells.

• 

  WB analysis using 17737-1-AP

  HeLa cells and HepG2 cells were subjected to SDS PAGE followed by western blot with 17737-1-AP (EDC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 17737-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17737-1-AP (EDC4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17737-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17737-1-AP (EDC4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 17737-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17737-1-AP (EDC4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 17737-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17737-1-AP (EDC4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 17737-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EDC4 antibody (17737-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 17737-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EDC4 antibody (17737-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IHC detected in: human liver cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 5 publications below

Product Information

17737-1-AP targets EDC4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EDC4 fusion protein Ag11784
• Full Name: enhancer of mRNA decapping 4
• Calculated Molecular Weight: 1401 aa, 152 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC064567
• Gene Symbol: EDC4
• Gene ID (NCBI): 23644
• RRID: AB_10665813
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Nat Cell Biol; O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins; Authors - Yulin Chen
human	WB	Mol Ther Nucleic Acids; The RNA binding protein QKI5 suppresses ovarian cancer via downregulating transcriptional coactivator TAZ.; Authors - Tao Liu
mouse	WB	J Genet Genomics; LSM14B coordinates protein component expression in the P-body and controls oocyte maturation; Authors - Huiru Zhang
human	IF	FEBS Lett; Gemin5 plays a role in unassembled-U1 snRNA disposal in SMN-deficient cells.; Authors - Dongxu Jiang
human	WB,IF	Res Sq; A protein-proximity screen reveals Ebola virus co-opts the mRNA decapping complex through the scaffold protein EDC4; Authors - Robert Davey ; KD Validated
mouse	WB	J Cell Biochem; NUSAP1 regulates mouse oocyte meiotic maturation; Authors - Lina Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Elisa (Verified Customer) (03-01-2022)

RPE1 cells stained for Hoechst (DNA marker, in green), EDC4 (mRNA decapping protein 4 in (P)-bodies, in magenta) and NEDD1 (pericentriolar matrix marker, in yellow). RPE1 cells were fixed in 4%PFA for 15’. Cells were then washed with PBS. Membrane permeabilization was then performed with 0.3% Triton for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Human retinal pigment epithelial-1 (RPE-1)

EDC4 Antibody Immunofluorescence validation (1:200 dilution) in Human retinal pigment epithelial-1 (RPE-1) (Cat no:17737-1-AP)

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