EDC3 Polyclonal antibody

EDC3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16486-1-AP

Synonyms

EDC3, hYjeF_N2, LSM16, LSM16 homolog, YJDC, YjeF_N2, YJEFN2

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Validation Data Gallery

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  WB analysis using 16486-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16486-1-AP (EDC3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16486-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16486-1-AP (EDC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16486-1-AP

  IP result of anti-EDC3 (IP:16486-1-AP, 4ug; Detection:16486-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human kidney using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16486-1-AP (EDC3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16486-1-AP (EDC3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16486-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16486-1-AP (EDC3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 16486-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EDC3 antibody (16486-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293T cells, MCF-7 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human kidney tissue, human heart tissue, human placenta tissue, human skin tissue, human liver tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

16486-1-AP targets EDC3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EDC3 fusion protein Ag9608
• Full Name: enhancer of mRNA decapping 3 homolog (S. cerevisiae)
• Calculated Molecular Weight: 508 aa, 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC021271
• Gene Symbol: EDC3
• Gene ID (NCBI): 80153
• RRID: AB_2095946
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	PLoS Genet; Two RNA binding proteins, ADAD2 and RNF17, interact to form a heterogeneous population of novel meiotic germ cell granules with developmentally dependent organelle association; Authors - Lauren G Chukrallah
rat	WB	Cell Rep; Identification of dynamic RNA-binding proteins uncovers a Cpeb4-controlled regulatory cascade during pathological cell growth of cardiomyocytes.; Authors - Eva Riechert
human		Nucleic Acids Res; RNA helicase DDX6 and scaffold protein GW182 in P-bodies promote biogenesis of stress granules; Authors - Vladimir Majerciak