DIXDC1 Polyclonal antibody

DIXDC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13816-1-AP

Synonyms

CCD1, Coiled coil DIX1, Coiled coil protein DIX1, DIX domain containing 1, DIXDC1, Dixin, KIAA1735

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 13816-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13816-1-AP (DIXDC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 13816-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13816-1-AP (DIXDC1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 13816-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13816-1-AP (DIXDC1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 13816-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13816-1-AP (DIXDC1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human ovary using 13816-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 13816-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 13816-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 13816-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 13816-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 13816-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 13816-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 13816-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 13816-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 13816-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, mouse heart tissue
• Positive IHC detected in: human heart tissue, human kidney tissue, human testis tissue, human skin tissue, human lung tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13816-1-AP targets DIXDC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DIXDC1 fusion protein Ag4904
• Full Name: DIX domain containing 1
• Calculated Molecular Weight: 683 aa, 77 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC033034
• Gene Symbol: DIXDC1
• Gene ID (NCBI): 85458
• RRID: AB_2091188
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Mol Neurobiol; Wnt Signaling Pathways Are Dysregulated in Rat Female Cerebellum Following Early Methyl Donor Deficiency.; Authors - Jérèmy Willekens