DDX5 Polyclonal antibody

DDX5 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 26385-1-AP

Synonyms

DDX5, DEAD box protein 5, G17P1, HELR, HLR1, HUMP68, p68, RNA helicase p68

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Validation Data Gallery

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  WB analysis of HepG2 using 26385-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26385-1-AP (DDX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26385-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26385-1-AP (DDX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 26385-1-AP

  IP result of anti-DDX5 (IP:26385-1-AP, 4ug; Detection:26385-1-AP 1:500) with HeLa cells lysate 1600 ug.

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  IF Staining of HepG2 using 26385-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX5 antibody (26385-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

26385-1-AP targets DDX5 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

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• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDX5 fusion protein Ag24821
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: NM_004396
• Gene Symbol: DDX5
• Gene ID (NCBI): 1655
• RRID: AB_2880498
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Eur J Med Chem; Discovery of potent thiazolidin-4-one sulfone derivatives for inhibition of proliferation of osteosarcoma in vitro and in vivo; Authors - Xuwen Chen