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INT11 Polyclonal antibody

INT11 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 15860-1-AP

Synonyms

CPSF3 like protein, CPSF3L, CPSF73L, INT11, Integrator complex subunit 11, INTS11, RC 68, RC68

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse colon tissue using INT11 Polyclonal antibody (15860-1-AP)

    WB analysis of mouse colon using 15860-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 15860-1-AP (INT11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using INT11 Polyclonal antibody (15860-1-AP)

    WB analysis of mouse spleen using 15860-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15860-1-AP (INT11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using INT11 Polyclonal antibody (15860-1-AP)

    IF Staining of HepG2 using 15860-1-AP

    Immunofluorescent analysis of HepG2 cells using 15860-1-AP (INT11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse colon tissue, mouse spleen tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15860-1-AP targets INT11 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INT11 fusion protein Ag8365
Full Name cleavage and polyadenylation specific factor 3-like
Calculated Molecular Weight 600 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC007978
Gene Symbol CPSF3L
Gene ID (NCBI) 54973
RRIDAB_2084537
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrator complex subunit 11 (INT11) is a protein that in humans is encoded by the INTS11 gene. INTS11, also known as Cleavage and Polyadenylation Specific Factor 3-Like (CPSF3L), contains a metallo-β-lactamase and β-CASP domain that, together, coordinate two zinc ions within the active site to promote its RNA endonuclease activity. INTS11 is the catalytic subunit of the Integrator (INT) complex, which is crucial for the biogenesis of small nuclear RNAs and enhancer RNAs. INT11 has 5 isoforms with the molecular mass of 56 and 65-68 kDa (PMID: 34516911, PMID: 37054711).

Protocols

Product Specific Protocols
WB protocol for INT11 antibody 15860-1-APDownload protocol
IF protocol for INT11 antibody 15860-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Britt (Verified Customer) (05-03-2023)

Antibody works well and is specific to target. Got a nice clear band.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HuH7