CLYBL Polyclonal antibody

CLYBL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17314-1-AP

Synonyms

citrate lyase beta like, CLB, CLYBL

---

Validation Data Gallery

• 

  WB analysis using 17314-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17314-1-AP (CLYBL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17314-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17314-1-AP (CLYBL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 17314-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 17314-1-AP (CLYBL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 17314-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17314-1-AP (CLYBL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17314-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17314-1-AP (CLYBL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HepG2 cells, human heart tissue, Jurkat cells, THP-1 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

17314-1-AP targets CLYBL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CLYBL fusion protein Ag11283
• Full Name: citrate lyase beta like
• Calculated Molecular Weight: 340 aa, 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC034360
• Gene Symbol: CLYBL
• Gene ID (NCBI): 171425
• RRID: AB_2083483
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLYBL, also named as CLB, is a Mitochondrial citramalyl-CoA lyase indirectly involved in the vitamin B12 metabolism (PubMed:29056341). It is a human mitochondrial enzyme of unknown function that is found in multiple eukaryotic taxa and conserved to bacteria. It acts as a beta-methylmalate synthase in vitro, by mediating conversion of glyoxylate and propionyl-CoA to beta-methylmalate. There're two isoforms with MW 31-33 kDa and 35-37 kDa.

Publications

Species	Application	Title
mouse	WB	Eur J Immunol; YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice.; Authors - Satabdi Nandi