CHD9 Polyclonal antibody

• Featured Product
• KD/KO Validated

CHD9 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ChIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 13402-1-AP

Synonyms

AD013, ATP dependent helicase CHD9, CHD 9, CHD9, CReMM, KIAA0308, KISH2, Kismet homolog 2, PRIC320, x0008

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Validation Data Gallery

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  WB analysis using 13402-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13402-1-AP (CHD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 13402-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13402-1-AP (CHD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 13402-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13402-1-AP (CHD9 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 13402-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13402-1-AP (CHD9 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HeLa using 13402-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13402-1-AP (CHD9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SGC-7901 cells, K-562 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• ChIP: See 1 publications below

Product Information

13402-1-AP targets CHD9 in WB, IF, IHC, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHD9 fusion protein Ag4245
• Full Name: chromodomain helicase DNA binding protein 9
• Calculated Molecular Weight: 2897 aa, 326 kDa
• Observed Molecular Weight: 330 kDa
• GenBank Accession Number: BC033770
• Gene Symbol: CHD9
• Gene ID (NCBI): 80205
• RRID: AB_10640425
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB, IHC	Mol Carcinog; miR-200c-3p spreads invasive capacity in human oral squamous cell carcinoma microenvironment.; Authors - Tomoyo Kawakubo-Yasukochi ; KD Validated
human	IHC	Braz J Med Biol Res; Decreased expression of chromodomain helicase DNA-binding protein 9 is a novel independent prognostic biomarker for colorectal cancer.; Authors - Li Xu
human	IHC	Int J Clin Exp Pathol; High CHD9 expression is associated with poor prognosis in clear cell renal cell carcinoma.; Authors - Bo Guan
human	WB,ChIP	mBio; A Two-Color Haploid Genetic Screen Identifies Novel Host Factors Involved in HIV-1 Latency.; Authors - Michael Röling ; KD Validated