CEP70 Polyclonal antibody

CEP70 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16280-1-AP

Synonyms

BITE, centrosomal protein 70kDa, Centrosomal protein of 70 kDa, CEP70, p10 binding protein

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Validation Data Gallery

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  WB analysis of human placenta using 16280-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16280-1-AP (CEP70 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 16280-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16280-1-AP (CEP70 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 16280-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16280-1-AP (CEP70 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary using 16280-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16280-1-AP (CEP70 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 16280-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16280-1-AP (CEP70 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 16280-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16280-1-AP (CEP70 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human placenta tissue, mouse testis tissue, PC-3 cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

16280-1-AP targets CEP70 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEP70 fusion protein Ag9365
• Full Name: centrosomal protein 70kDa
• Calculated Molecular Weight: 597 aa, 70 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: BC016050
• Gene Symbol: CEP70
• Gene ID (NCBI): 80321
• RRID: AB_2077083
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP70, also named as Centrosomal protein of 70 kDa, is a 597 amino acid protein, which directly interacts with tubulin-gamma; this interaction determines centrosomal localization. CEP70 plays a role in the organization of both preexisting and nascent microtubules in interphase cells. During mitosis, CEP70 is required for the organization and orientation of the mitotic spindle. CEP70 exists some isoforms with MW 70, 67, 65 and 25 kDa.

Publications

Species	Application	Title
human,mouse	IF	Front Endocrinol (Lausanne); Identification of biallelic variations of CEP70 in patients with male infertility; Authors - Tiechao Ruan