CDC20B Polyclonal antibody

CDC20B Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13376-1-AP

Synonyms

CDC20B, G6VTS76519

---

Validation Data Gallery

• 

  IHC staining of human kidney using 13376-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13376-1-AP (CDC20B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 13376-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13376-1-AP (CDC20B antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 13376-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13376-1-AP (CDC20B antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

13376-1-AP targets CDC20B in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDC20B fusion protein Ag4198
• Full Name: cell division cycle 20 homolog B (S. cerevisiae)
• Calculated Molecular Weight: 192 aa, 22 kDa
• GenBank Accession Number: BC037547
• Gene Symbol: CDC20B
• Gene ID (NCBI): 166979
• RRID: AB_2877945
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse,human	WB,IF,CoIP	Nat Commun; CDC20B is required for deuterosome-mediated centriole production in multiciliated cells.; Authors - Diego R Revinski
human	IHC	Front Oncol; Identification of Hub Genes Associated With Melanoma Development by Comprehensive Bioinformatics Analysis.; Authors - Jie Jiang
human	WB	Bioengineered; Circular RNA hsa_circ_0008896 accelerates atherosclerosis by promoting the proliferation, migration and invasion of vascular smooth muscle cells via hsa-miR-633/CDC20B (cell division cycle 20B) axis.; Authors - Xumin Hou