Anti-Human CD73 (AD2) Rabbit Recombinant Antibody

CD73 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

AD2

Cat no : 65500-1-RR

Synonyms

5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), CD73, E5NT, Ecto 5 nucleotidase, eN, eNT, NT, NT5, NT5E, NTE

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Validation Data Gallery

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  FC experiment of human PBMCs using 65500-1-RR

  1X10^6 human PBMCs were surface stained with CL488 Anti-Human CD3 and 0.4 ug Anti-Human CD73 Rabbit Recombinant Antibody (65500-1-RR, Clone:AD2) or Rabbit IgG control PolyAb. Unconjugated antibodies were labeled with FlexAble CL750 Antibody Labeling Kit for Rabbit IgG (KFA004) prior to staining. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65500-1-RR

  1X10^6 human PBMCs were surface stained with 0.4 ug Anti-Human CD73 Rabbit Recombinant Antibody (65500-1-RR, Clone:AD2) or Rabbit IgG control PolyAb. Unconjugated antibodies were labeled with FlexAble CL750 Antibody Labeling Kit for Rabbit IgG (KFA004) prior to staining. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65500-1-RR

  1X10^6 human PBMCs were surface stained with CL488 Anti-Human CD3 and 0.4 ug Anti-Human CD73 Rabbit Recombinant Antibody (65500-1-RR, Clone:AD2) (left) or 0.4 ug Anti-Human CD73 Mouse Monoclonal Antibody (Clone:AD2) (center and right). Each unconjugated primary antibody was labeled with FlexAble CL750 Antibody Labeling Kit for Rabbit IgG (KFA004) or FlexAble CL750 Antibody Labeling Kit for Mouse IgG1 (KFA024) prior to staining. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65500-1-RR targets CD73 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Pre-B leukemia cell line
• Full Name: 5'-nucleotidase, ecto (CD73)
• Calculated Molecular Weight: 29 kDa, 63 kDa
• GenBank Accession Number: BC015940
• Gene Symbol: NT5E
• Gene ID (NCBI): 4907
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).