CC2D1A Monoclonal antibody

CC2D1A Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C11G2

Cat no : 68334-1-Ig

Synonyms

CC2D1A, FLJ20241, FLJ41160, FREUD 1, Freud 1/Aki1, MRT3

---

Validation Data Gallery

• 

  WB analysis using 68334-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68334-1-Ig (CC2D1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 68334-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68334-1-Ig (CC2D1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 68334-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CC2D1A antibody (68334-1-Ig, Clone: 1C11G2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Tested Applications

• Positive WB detected in: U2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68334-1-Ig targets CC2D1A in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CC2D1A fusion protein Ag10363
• Full Name: coiled-coil and C2 domain containing 1A
• Calculated Molecular Weight: 951 aa, 104 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC064981
• Gene Symbol: CC2D1A
• Gene ID (NCBI): 54862
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CC2D1A (coiled-coil and C2 domain-containing 1A), also known as Freud-1, Aki1 or TAPE (TBK1-associated protein in endolysosomes), is a evolutionary conserved protein located in different subcellular compartments, including the nucleus, centrosome, and endolysosomes. It acts as a scaffold protein that interacts with various proteins and plays diverse biological roles. Mutations in CC2D1A have been linked to nonsyndromic mental retardation (NMSR) and generate a truncated 85-kDa product. Several isoforms of CC2D1A proteins have also been described: 120 /130-kDa doublet of long isoform and 67 kDa short isoform. The short isoform of CC2D1A has been identified as the predominant isoform in rodent cells, while the long isoform is more abundant in human cells. Recently it has been reported that CC2D1A/TAPE is the first innate immune regulator implicated in both TLR and RLR signaling at a very early step.