CAP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CAP1 Polyclonal Antibody for WB, IP, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 16231-1-AP

Synonyms

Adenylyl cyclase associated protein 1, CAP, CAP 1, CAP1, CAP1 PEN

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Validation Data Gallery

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  WB analysis using 16231-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 16231-1-AP (CAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 16231-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16231-1-AP (CAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 16231-1-AP

  IP result of anti-CAP1 (IP:16231-1-AP, 3ug; Detection:16231-1-AP 1:500) with A431 cells lysate 1520ug.

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  IHC staining of human pancreas cancer using 16231-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16231-1-AP (CAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 16231-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16231-1-AP (CAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 16231-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16231-1-AP (CAP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 16231-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16231-1-AP (CAP1 Antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of HeLa using 16231-1-AP

  1X10^6 HeLa cells were stained with 0.2ug CAP1 antibody (16231-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: Caco-2 cells, COLO 320 cells, HepG2 cells
• Positive IP detected in: A431 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Product Information

16231-1-AP targets CAP1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CAP1 fusion protein Ag8876
• Full Name: CAP, adenylate cyclase-associated protein 1 (yeast)
• Calculated Molecular Weight: 475 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC013963
• Gene Symbol: CAP1
• Gene ID (NCBI): 10487
• RRID: AB_2172166
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAP1, also known as CAP, CAP1-PEN, belongs to the CAP family and contains 1 C-CAP/cofactor C-like domain. CAP1 binds to monomeric actin and regulates the actin cytoskeleton to control cell size, adhesion, and migration (PMID: 23737525). CAP1 also functions as a receptor for inflammatory cytokine resistin (PMID: 24606903). CAP1 is an abundant and ubiquitously expressed protein. Western blot analysis detected CAP1 at an apparent molecular mass of 52 kDa.

Publications

Species	Application	Title
rat	WB	Proc Natl Acad Sci U S A; CAP1 binds and activates adenylyl cyclase in mammalian cells.; Authors - Xuefeng Zhang
human	WB,IF	J Biol Chem; Cyclase-Associated Protein 1 (CAP1) is a prenyl-binding partner of Rap1 GTPase.; Authors - Xuefeng Zhang ; KD Validated
pig	WB	Viruses; Porcine Circovirus Type 2 Induces Single Immunoglobulin Interleukin-1 Related Receptor (SIGIRR) Downregulation to Promote Interleukin-1β Upregulation in Porcine Alveolar Macrophage.; Authors - Shunli Yang
bovine	WB	Cell Biochem Funct; Cyclase-associated protein 1 is a key negative regulator of milk synthesis and proliferation of bovine mammary epithelial cells.; Authors - Xiaohan Yuan ; KD Validated
mouse	WB	Cell Mol Life Sci; Functional interdependence of the actin regulators CAP1 and cofilin1 in control of dendritic spine morphology; Authors - Anika Heinze