CEP85L Polyclonal antibody

• Featured Product
• KD/KO Validated

CEP85L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24588-1-AP

Synonyms

C6orf204, CEP85L, NY BR 15

---

Validation Data Gallery

• 

  WB analysis of PC-13 using 24588-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 24588-1-AP (C6orf204 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 24588-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24588-1-AP (CEP85L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 24588-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24588-1-AP (C6orf204 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 24588-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24588-1-AP (C6orf204 Antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: PC-13 cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:300-1:800
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 24588-1-AP is CEP85L Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEP85L fusion protein Ag20235
• Full Name: chromosome 6 open reading frame 204
• Calculated Molecular Weight: 805 aa, 92 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC153054
• Gene Symbol: C6orf204
• Gene ID (NCBI): 387119
• RRID: AB_2819012
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP85L, also named as C60rf204, is a gene which encodes a protein that has identified as a breast cancer antigen. Nothing more is known of its function at this time. There're five transcript variants with MW 45 kDa, 55-56 kDa and 91-92 kDa.

Publications

Species	Application	Title
mouse	WB,IF	Neuron; Pathogenic Variants in CEP85L Cause Sporadic and Familial Posterior Predominant Lissencephaly.; Authors - Meng-Han Tsai
human	WB,IF,CoIP	Neuron; Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.; Authors - Andrew Kodani ; KD Validated