C20orf3/APMAP Polyclonal antibody

• Featured Product
• KD/KO Validated

C20orf3/APMAP Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25953-1-AP

Synonyms

APMAP, BSCv, C20orf3, C20orf3/APMAP, Protein BSCv

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Validation Data Gallery

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  WB analysis of HepG2 using 25953-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25953-1-AP (C20orf3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 25953-1-AP

  IP result of anti-C20orf3/APMAP (IP:25953-1-AP, 4ug; Detection:25953-1-AP 1:300) with mouse liver tissue lysate 4800ug.

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  IHC staining of human kidney using 25953-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25953-1-AP (C20orf3 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 25953-1-AP is C20orf3/APMAP Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C20orf3/APMAP fusion protein Ag23029
• Full Name: chromosome 20 open reading frame 3
• Calculated Molecular Weight: 416 aa, 46 kDa
• Observed Molecular Weight: 42-46 kDa
• GenBank Accession Number: BC003501
• Gene Symbol: C20orf3
• Gene ID (NCBI): 57136
• RRID: AB_2880308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC,IF	Cancer Res; Cholesterol induces epithelial-to-mesenchymal transition of prostate cancer cells by suppressing degradation of EGFR through APMAP.; Authors - Siyuan Jiang ; KD Validated
mouse	WB	J Nutr Biochem; Thioredoxin domain containing 5 is involved in the hepatic storage of squalene into lipid droplets in a sex-specific way; Authors - Javier Sánchez-Marco