Hikeshi Polyclonal antibody

• Featured Product
• KD/KO Validated

Hikeshi Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 14808-1-AP

Synonyms

C11orf73, Hikeshi, HSPC138, HSPC179, HSPC248, L7RN6

---

Validation Data Gallery

• 

  WB analysis of mouse kidney using 14808-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14808-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 14808-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 14808-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14808-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14808-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 14808-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14808-1-AP (Hikeshi antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14808-1-AP

  IP result of anti-Hikeshi (IP:14808-1-AP, 4ug; Detection:14808-1-AP 1:500) with mouse brain tissue lysate 2640ug.

• 

  IHC staining of human kidney using 14808-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14808-1-AP (Hikeshi antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14808-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14808-1-AP (Hikeshi antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse kidney tissue, A375 cells, mouse brain tissue, PC-3 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

14808-1-AP targets Hikeshi in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Hikeshi fusion protein Ag6530
• Full Name: chromosome 11 open reading frame 73
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC001677
• Gene Symbol: C11orf73
• Gene ID (NCBI): 51501
• RRID: AB_10644295
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
		Oncogene; HSP110 promotes colorectal cancer growth through STAT3 activation.; Authors - K Berthenet
human	WB	J Med Genet; Leukoencephalopathy and early death associated with an Ashkenazi-Jewish founder mutation in the Hikeshi gene.; Authors - Simon Edvardson
human	WB	Int J Mol Med; HIKESHI silencing can enhance mild hyperthermia sensitivity in human oral squamous cell carcinoma HSC‑3 cells.; Authors - Yoshiaki Tabuchi ; KD Validated
human,mouse	WB,IHC	Pharmaceutics; Role of HIKESHI on Hyperthermia for Castration-Resistant Prostate Cancer and Application of a Novel Magnetic Nanoparticle with Carbon Nanohorn for Magnetic Hyperthermia; Authors - Takashi Nagai ; KD Validated
mouse	WB	Front Genet; Nuclear Accumulation of HSP70 in Mouse Skeletal Muscles in Response to Heat Stress, Aging, and Unloading With or Without Reloading.; Authors - Antonios Apostolopoulos