BRCC3 Polyclonal antibody

BRCC3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15391-1-AP

Synonyms

BRCA1 A complex subunit BRCC36, BRCC3, BRCC36, BRISC complex subunit BRCC36, C6.1A, CXorf53

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Validation Data Gallery

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  WB analysis using 15391-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15391-1-AP (BRCC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human nasopharyngeal carcinoma using 15391-1-AP

  Immunohistochemical analysis of paraffin-embedded human nasopharyngeal carcinoma tissue slide using 15391-1-AP (BRCC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, PC-3 cells
• Positive IHC detected in: human nasopharyngeal carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

15391-1-AP targets BRCC3 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BRCC3 fusion protein Ag7584
• Full Name: BRCA1/BRCA2-containing complex, subunit 3
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC002999
• Gene Symbol: BRCC3
• Gene ID (NCBI): 79184
• RRID: AB_2092136
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRCA1-BRCA2-containing complex subunit 3 (BRCC3), a Lys-63-specific deubiquitinase, is a member of the JAMM/MPN family of zinc metalloproteases. BRCC3 have been shown to promote the inflammasome activation by deubiquitinating NOD-like receptor containing pyrin domain 3 (NLRP3). BRCC3 is associated with G2/M arrest in breast cancer cells and DNA damage. The expression of BRCC3 is associated with increased cell proliferation. (PMID: 23246432, PMID: 30272359)

Publications

Species	Application	Title
pig	WB	Mol Cell Proteomics; The mechano-ubiquitinome of articular cartilage: Differential ubiquitination and activation of a group of ER-associated DUBs and ER stress regulators; Authors - Nitchakarn Kaokhum
human	IHC	Transl Oncol; A Novel Scoring Model of Deubiquitination Patterns Predicts Prognosis and Immunotherapeutic Response in Hepatocellular Carcinoma; Authors - Jiahao Zhang
mouse	WB,CoIP	J Agric Food Chem; Methionine Promotes Milk Synthesis through the BRCC36-BRG1-mTOR Signaling Axis in Mammary Epithelial Cells; Authors - Liping Xie ; KD Validated